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        產(chǎn)品詳情
        • 產(chǎn)品名稱: MC/CAR

        • 產(chǎn)品型號(hào):CRL-8083
        • 產(chǎn)品廠商:美國(guó)標(biāo)準(zhǔn)生物品收藏中心(ATCC)
        • 產(chǎn)品價(jià)格:0
        • 折扣價(jià)格:0
        • 產(chǎn)品文檔:
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        簡(jiǎn)單介紹:
        CRL-8083 MC/CAR 人外周血LIN巴細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
        詳情介紹:
        CRL-8083 MC/CAR 人外周血**細(xì)胞
        ATCC® Number: CRL-8083?    Price: $417.00
        Designations: MC/CAR
        Depositors:  Research Corporation
        Isotype: IgG1 (cytoplasmic); kappa light chain
        Biosafety Level: 2
        Shipped: frozen
        Medium & Serum: See Propagation
        Growth Properties: suspension
        Organism: Homo sapiens (human)
        Morphology: lymphoblast

        Source: Organ: peripheral blood
        Disease: plasmacytoma; myeloma
        Cell Type: B lymphocyte;
        Cellular Products: immunoglobulin
        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
         
        Receptors: complement (C3b)
        Tumorigenic: Yes
        Antigen Expression: HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
        DNA Profile (STR): Amelogenin: X,Y
        CSF1PO: 11,12
        D13S317: 11,13
        D16S539: 11,12
        D5S818: 11,14
        D7S820: 9,13
        THO1: 6,9
        TPOX: 8
        vWA: 17,18
        Cytogenetic Analysis: normal male diploid
        Isoenzymes: ADA, 1
        AK-1, 1
        ES-D, 1
        G6PD, B
        GLO-I, 1
        PGM1, 1
        PGM3, 1
        Age: 81 years
        Gender: male
        Ethnicity: Caucasian
        Comments: Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]
        The cells produce, but do not secrete, immunoglobulin.
        No surface immunoglobulin.
        8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
        Can be used as a human fusion line.
        Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
        Temperature: 37.0°C
        Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.
        References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984
        43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
        43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
        58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719
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