In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli.
Propagation:
ATCC complete growth medium: A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium with 10 mM HEPES and 2.0 g/L sodium bicarbonate supplemented with 0.005 mg/ml insulin and 10 fetal bovine serum Temperature: 37.0°C
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25 trypsin, 0.03 EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:
culture medium 95; DMSO, 5
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References:
2151: Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371 22666: Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137 29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 32565: Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448
