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SNU-1
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J Park
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Biosafety Level:
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1
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frozen
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See Propagation
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suspension, multicell aggregates
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Homo sapiens (human)
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epithelial
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Organ: stomach
Disease: gastric carcinoma
Derived from metastatic site: ascites
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: 1984
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vasoactive intestinal peptide (VIP), expressed
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myc +; erb B2 +
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Blood Type O; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
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Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11,14
D16S539: 9,12
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,10
vWA: 14
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SNU-1 is a near-diploid cell line with a modal chromosome number of 47; double minute chromosomes were found in 28% of the cells. They have a reported colony-forming efficiency of 1.9% in semisolid medium and a population doubling time of 26 hours. The cells grow as floating aggregates of round cell clusters. This is a hyperdiploid human cell line with the modal chromosome number of 47 occurring in 94% of cells. Higher ploidies occurred at 42%, which is very high. The karyotype of 47,XY,t(1;4) (q24;q33), +M1 is very uniform and stable. M1 is an F- sized small metacentric chromosome.
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44 years *****
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male
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Asian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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26 hrs
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
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23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397
23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
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