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產(chǎn)品名稱：CHP 4 人乳腺癌細(xì)胞
產(chǎn)品型號：CCL-133
產(chǎn)品廠商：美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品價(jià)格：0
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簡單介紹：

CHP 4 人乳腺癌細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！

詳情介紹：

CHP 4 人乳腺癌細(xì)胞

ATCC^® Number:

CCL-133?

Price:

$399.00

Designations:	CHP 4 (W.W.)
Depositors:	WJ Mellman
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)

Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C.
Preservation:	Freeze medium: complete growth medium, 95%; DMSO, 5%

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