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        產品詳情
        • 產品名稱:TT 人甲狀腺導管癌細胞

        • 產品型號:CRL-1803
        • 產品廠商:美國標準生物品收藏中心(ATCC)
        • 產品價格:0
        • 折扣價格:0
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        CRL-1803 TT 人甲狀腺導管癌細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!
        詳情介紹:
        CRL-1803 TT 人甲狀腺導管癌細胞
        ATCC® Number: CRL-1803?    Price:  
        Designations: TT
        Depositors:  SS Leong
        Biosafety Level: 1
        Shipped: frozen
        Medium & Serum: See Propagation
        Growth Properties: adherent
        Organism: Homo sapiens (human)
        Morphology: epithelial

        Source: Organ: thyroid
        Tissue: medulla
        Disease: carcinoma
        Cellular Products: calcitonin; carcinoembryonic antigen (CEA) [21867]
        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
         
        Tumorigenic: Yes
        DNA Profile (STR): Amelogenin: X
        CSF1PO: 10,13
        D13S317: 11
        D16S539: 12,13
        D5S818: 12,13
        D7S820: 10,12
        THO1: 6,9
        TPOX: 8,11
        vWA: 16,18
        Cytogenetic Analysis: aneuploid with several markers; modal number = 40; range = 32 to 86 [21867]
        Age: 77 years
        Gender: female
        Ethnicity: Caucasian
        Comments: The TT cell line was established by S.S. Leong, et al. from a specimen obtained by needle biopsy from a 77 year old female with thyroid medullary carcinoma. [21867]
        TT cells continuously produce high levels of calcitonin and CEA. [21867]
        Immunoreactive calcitonin was found to be produced in cell culture at levels of 3900 pg/million cells and 7700 pg/million cells 24 and 72 hours respectively after a medium change. [21867]
        CEA was found to accumulate to greater than 27 ng/million cells over a 72 hours period. [21867]
        Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes. [21867]
        The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1 mM L-glutamine.
        It is not known if the neuropeptides reported to be produced by this cell line when it was grown in RPMI 1640 medium are also produced by the cells when they are cultured in Ham's F12K medium.
        Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
        Temperature: 37.0°C
        Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
        Medium Renewal: Twice per week
        Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
        Add fresh culture medium, aspirate and dispense into new culture flasks.
        Preservation: Culture medium, 95%; DMSO, 5%
        Doubling Time: 83 hrs
        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
        recommended serum:ATCC 30-2020
        References: 21867: . Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.
        32264: Behr TM, et al. Improved treatment of medullary thyroid cancer in a nude mouse model by combined radioimmunochemotherapy: doxorubicin potentiates the therapeutic efficacy of radiolabeled antibodies in a radioresistant tumor type. Cancer Res. 57: 5309-5319, 1997. PubMed: 9393755
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