CCL-171 MRC-5 人胚肺細(xì)胞
ATCC® Number: CCL-171?
Designations: MRC-5
Depositors: JP Jacobs
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
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Isolation: Isolation date: September, 1966
Applications: efficacy testing [92587]
testing [92346] [92389]
transfection host (Roche FuGENE® Transfection Reagents)
viruscide testing [92579] [92582]
Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15
Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.
Isoenzymes: G6PD, B
Age: 14 weeks gestation
Gender: male
Ethnicity: Caucasian
Comments: The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966.
The cells are capable of 42 to 46 population doublings before the onset of senescence.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
