CCL-240 HL-60 人原髓細胞白血病細胞
ATCC® Number: CCL-240?
Designations: HL-60
Depositors: RC Gallo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: myeloblastic
Source: Organ: peripheral blood
Disease: acute promyelocytic leukemia
Cell Type: promyeloblast;
Cellular Products: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid [23403]
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Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Receptors: complement, expressed [1050]
Fc, expressed [1050]
Tumorigenic: Yes
Reverse Transcript: negative
Oncogene: myc +
DNA Profile (STR): Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7,8
TPOX: 8,11
vWA: 16
Cytogenetic Analysis: The stemline chromosome number is pseudodiploid with the 2S component occurring at 6.2%. Five markers (M2 through M6) were common to most S metaphases. DM's, which varied in numbers per cell, occurred in all metaphases karyotyped. HSR chromosomes were not detected.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1
PGM1, 1
PGM3, 1
Age: 36 years
Gender: female
Ethnicity: Caucasian
Comments: HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. [22902]
HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. [1229]
The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. [1050]
The line is positive for myc oncogene expression.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
purified DNA:ATCC CCL-240D
purified RNA:ATCC CCL-240R
References: 1050: Gallagher R, et al. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713-733, 1979. PubMed: 288488
1229: Collins SJ, et al. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. Natl. Acad. Sci. USA 75: 2458-2462, 1978. PubMed: 276884
22902: Collins SJ, et al. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature 270: 347-349, 1977. PubMed: 271272
23403: Aggarwal BB, et al. Human tumor necrosis factor. Production, purification, and characterization. J. Biol. Chem. 260: 2345-2354, 1985. PubMed: 3871770
32237: Nahm MH, et al. Identification of cross-reactive antibodies with low opsonophogocytic activity for Streptoccus pneumoniae. J. Infect. Dis. 176: 698-703, 1997. PubMed: 9291318
32253: Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed: 9284127
32256: Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636
32277: Mansat V, et al. The protein kinase C activators phorbol esters and phosphatidylserine inhibit neutral aphingomyelinase activation, ceramide generation, and apoptosis triggered by daunorubicin. Cancer Res. 57: 5300-5304, 1997. PubMed: 9393753
32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57:
