HB-8065 Hep G2 人肝癌細胞系
ATCC® Number: HB-8065?
Designations: Hep G2
Depositors: Wistar Institute
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: liver
Disease: hepatocellular carcinoma
Cellular Products: alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; [3525]
complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein) [3525]
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Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Receptors: insulin; insulin-like growth factor II (IGF II) [22446]
Tumorigenic: No
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10
F13A01: 5,7
F13B: 6,10
FESFPS: 11
LPL: 10,11
THO1: 9
TPOX: 8,9
vWA: 17
Cytogenetic Analysis: modal number = 55 (range = 50 to 60); has a rearranged chromosome 1 [3525]
Age: 15 years adolescent
Gender: male
Ethnicity: Caucasian
Comments: The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities. [23557]
The cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress). [26594]
There is no evidence of a Hepatitis B virus genome in this cell line. [1205] [22909]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
derivative:ATCC CRL-10741
derivative:ATCC CRL-11997
purified DNA:ATCC HB-8065D
References: 1205: Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960
3525: Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
22446: Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141
22909: Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137
23557: Busch SJ, et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase
