HeLa 229 人宮頸癌細胞
ATCC® Number: CCL-2.1? Price: $323.00
Designations: HeLa 229
Depositors: JT Syverton
Biosafety Level: 2 [Cells contain human papilloma virus (HPV-18) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent (The line can be readily adapted to growth in suspension)
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: cervix
Disease: adenocarcinoma
Cellular Products: keratin
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Virus Susceptibility: Human adenovirus 7
Vesicular stomatitis virus
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes: G6PD, A
Age: 31 years
Gender: female
Ethnicity: Black
HeLa Markers: Y
Comments: Relatively resistant to polioviruses.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
